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  18. E1. A method of testing or determining in vivo
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    in neuronal cells which are in contact with the molecule.
    The present disclosure provides a method of testing or determining
    in vivo acute neurotoxicity of a molecule comprising measuring oscillations in intracellular free calcium concentration (“calcium oscillations”) in vitro in neuronal
    cells which are in contact with the molecule.
    The present methods can also improve efficiency (i.e.,
    shorten) the evaluation period of candidate molecules by reducing the
    screening time period for selection of molecules that do not
    exhibit in vivo acute neurotoxicity. The present disclosure provides methods for testing
    or determining the toxicity (e.g., in vivo acute neurotoxicity) of
    a molecule by measuring certain characteristics of
    the molecule. The present invention relates to methods for
    selecting therapeutic molecules that have reduced toxic side
    effects. In yet other embodiments, a therapeutic molecule of the invention binds to a molecule which is
    useful in treating a neurological disease or disorder.
    The above methods of the present invention can further comprise measuring tubulin intensity in a culture of neuronal cells, expression of a target protein,
    or behavioral performance of the molecule. The present disclosure also
    provides methods for selecting a molecule having reduced toxic side effects.

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